Two works related to the effect of Blue Light irradiation on cells, specifically on fibroblasts, have been published in the Biomedicine journal Special Issue “Cellular Mechanism in Wound Healing”, edited by Stefano Bacci. The articles report the results of the research activity developed in the last years by the CNR - Institute of Applied Physics Nello Carrara in collaboration with the European Laboratory for Non Linear Spectroscopy, the University of Florence, the City of Health and Science (Tourin) and Emoled, to investigate the Blue Light mechanism of action as emerging therapy for the management of hard-to-heal wound and as potential treatment for keloids prevention. Congratulations to Francesca Rossi, Giada Magni and all the research team for this achievement.

 The results of the two studies are briefly presented below.

Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing.

The goal of the study was to investigate the effect of Blue Light at cellular level and at different doses; the selected cellular targets: fibroblasts derived from human healthy tissue and cultured keratinocytes cells. Tests performed on cell metabolism and proliferation at different doses evidenced that lower doses induce an enhancement in fibroblasts activity while higher doses are inhibitory (biphasic dose response). Raman Micro-Spectroscopy analysis was performed on cellular targets before and after irradiation with two different doses of light. Significant spectral variations related to a change in the redox state of Cytocrome C were registered in fibroblasts. Cytocrome C is one of the most important photoacceptors of visible light and the earliest molecule involved in the trigger of the Photobiomodulation. This event is time-dependent - it is immediately activated and it is more evident after 20 minutes in all fibroblasts – suggesting an amplification of the signal after the initial stimulus, typical of an ongoing cellular response. According to the authors the biphasic dose response of fibroblasts and the induced changes in their redox state confirm the hypothesis that Blue Light is able to induce Photobiomodulation in wounds: it transfers the energy to the wound area thus resulting in a photochemical effect possibly modulating fibroblasts activity and promoting the wound healing process.

 Link to the article: https://bit.ly/3qbDULB

Experimental Study on Blue Light Interaction with Human Keloid-Derived Fibroblasts.

 In the study the effects of different doses of Blue LED Light on fibroblasts isolated from keloids and perilesional tissue were investigated to point out whether Photobiomodulation with Blue Light can be applied in the treatment of keloid scars and to prevent their reappearance. Results confirm that the Blu Light can modulate both cell metabolism and proliferation, after 24 h only, in a dose-dependent manner, in either type of fibroblasts. The Raman Micro-spectroscopy analysis demonstrated that Blue Light modulate the redox state of Cytochrome C also in keloid and perilesional fibroblasts, confirming the responsiveness of this molecule to the Blue Light excitation, and showing a different behavior in keloids. According to the authors Photobiomodulations with Blue Light could be an innovative and minimally invasive approach to the management of hypertrophic scars and keloids, in association with current treatments.

Link to the article: https://bit.ly/3bprPhs